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Author(s):	Hofbauer, Wolfgang Karl
Title:
Abstract:	Bei mikrobiellen Untersuchungen von verschiedenen biogenen schwarzen Verfärbungen von Wasserleitungssystemen oder damit verbundenen Einrichtungen wurden unterschiedliche Mikroorganismen festgestellt. Überraschend häufig wurden dabei freilebende Amöben (FLA), insbesonders aus der Gattung Acanthamoeba, beobachtet, die zusammen mit Bakterien und schwarzen Hefen auftraten. Die untersuchten Biofilme legen in dreifacher Hinsicht eine potenzielle hygienische Belastung nahe: das Auftreten potenziell pathogener Amöben, die wahrscheinliche Beteiligung von pathogenen Bakterien und das Vorkommen von opportunistischen Pilzen. Eine charakteristische Kombination von Umweltfaktoren führt zur Entwicklung dieser speziellen Biofilme, nämlich hohe Temperaturen, periodische Feuchte und damit verbundener Wasserstress oder hoher osmotischer Druck bzw. Salzgehalt sowie die Anwendung von Reinigungsmitteln und fallweise von Desinfektionsmitteln. Amoebae, bacteria and fungi containing biofilms occuring in the built environment Microbial investigations of various organic black stains from water systems or connected facilities revealed different constituting microorganisms. Surprisingly regularly, free-living amoebae (FLA), especially of the genus Acanthamoeba, together with bacteria and black yeasts were found. Altogether we recognized a potentially three-fold hygienic load emanating from the investigated biofilms which are potentially pathogenic amoebae, the likely involvement of pathogenic bacteria and the presence of opportunistic fungi. Furthermore we realized that the conditions at which such biofilms developed are characterized by a typical combination of environmental factors. These are high temperature, potentially intermittent wetness and connected water stress or high osmotic pressure/salinity and the application of detergents and occassionally disinfectants.
Source:	Bauphysik 40 (2018), No. 5
Page/s:	396-404
Language of Publication:	German

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